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Corning Life Sciences 96-well costar flat-bottom luminometer plates
96 Well Costar Flat Bottom Luminometer Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/96-well+costar+flat-bottom+luminometer+plates/96+well+costar+flat+bottom+luminometer+plates/pm40168909-332-47-49
Average 90 stars, based on 1 article reviews
96-well costar flat-bottom luminometer plates - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Luciferase:

Article Title: Structure-based discovery of Middle East respiratory syndrome coronavirus fusion inhibitor
Article Snippet: The culture was re-fed with fresh medium 12 h post-infection and incubated for an additional 72 h. Cells were washed with PBS and lysed using lysis reagent included in a luciferase kit (Promega). .. Aliquots of cell lysates were transferred to 96-well Costar flat-bottom luminometer plates (Corning Costar), followed by the addition of luciferase substrate (Promega). .. Relative light units were determined immediately in the Ultra 384 luminometer (Tecan US) .

Article Title: Dendritic-cell-targeting virus-like particles as potent mRNA vaccine carriers.
Article Snippet: Messenger RNA vaccines lack specificity for dendritic cells (DCs)—the most effective cells at antigen presentation.. Here we report the design and performance of a DC-targeting virus-like particle pseudotyped with an engineered Sindbis-virus glycoprotein that recognizes a surface protein on DCs, and packaging mRNA encoding for the Spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or for the glycoproteins B and D of herpes simplex virus 1.. Injection of the DC-targeting SARS-CoV-2 mRNA vaccine in the footpad of mice led to substantially higher and durable antigen-specific immunoglobulin-G titres and cellular immune responses than untargeted virus-like particles and lipid–nanoparticle formulations.

Article Title: A single dose SARS-CoV-2 simulating particle vaccine induces potent neutralizing activities
Article Snippet: .. Huh-7 cells were subsequently lysed with 50 μL lysis reagent (Promega), and 30 μL lysates were transferred to 96-well Costar flat-bottom luminometer plates (Corning Costar) for the detection of relative light units using the Firefly Luciferase Assay Kit (Promega) with an Ultra 384 luminometer (Tecan). .. A nonlinear regression analysis was performed on the resulting curves using Prism (GraphPad) to calculate half-maximal inhibitory concentration (EC50) values.

Article Title: A pan-coronavirus fusion inhibitor targeting the HR1 domain of human coronavirus spike
Article Snippet: .. Twelve hours after infection, culture medium was refreshed and then incubated for an additional 48 hours, followed by washing cells with PBS, lysing cells with lysis reagent (Promega), and transferring the cell lysates to 96-well Costar flat-bottom luminometer plates (Corning Costar) for the detection of relative light units using the Firefly Luciferase Assay Kit (Promega) and an Ultra 384 luminometer (Tecan). ..

Article Title: A potent SARS-CoV-2 antibody neutralizes Omicron variant by disassembling the spike trimer
Article Snippet: .. Huh-7 cells were subsequently lysing with 50 μL lysis reagent (Promega), and 30 μL of the lysates were transferred to 96-well Costar flat-bottom luminometer plates (Corning Costar) for the detection of relative light units using the Firefly Luciferase Assay Kit (Promega) on the instrument of an Ultra luminometer (Tecan). .. A nonlinear regression analysis was performed on the resulting curves using Prism (GraphPad) to calculate half-maximal inhibitory concentration (IC 50 ) values.

Article Title: ADS-J1 inhibits HIV-1 infection and membrane fusion by targeting the highly conserved pocket in the gp41 NHR-trimer.
Article Snippet: The cells were washed with PBS and lysed using the lysis reagent from a luciferase kit (Promega, Madison, WI). .. The cell lysates were transferred to 96-well Costar flat-bottom luminometer plates (Corning Costar, Corning, NY) and luciferase substrate (Promega) was added to the plate, followed by immediate measuring for luciferase activity (relative light units, RLU) using an Ultra 384 luminometer (Tecan, USA). .. To construct vectors encoding N36Fd and its mutants, the corresponding DNA fragments in the pseudovirus mutants Q64 (A/L) and A67 (G/S), as well as the T2635-resistant virus carrying a Q66R mutation, were amplified by PCR using the Platinum PCR SuperMix High Fidelity Kit (Invitrogen) with a forward primer containing a BamH1 site and a reverse primer coding the first 6 amino acids of the Fd fragment.

Article Title: Inefficiency of Sera from Mice Treated with Pseudotyped SARS-CoV to Neutralize 2019-nCoV Infection
Article Snippet: Then, the cells were washed with PBS and lysed with lysis reagent (Promega). .. The cell lysates were transferred to 96-well Costar flat-bottom luminometer plates (Corning Costar), followed by detection of relative light units using a Firefly Luciferase Assay Kit (Promega). ..

Lysis:

Article Title: Dendritic-cell-targeting virus-like particles as potent mRNA vaccine carriers.
Article Snippet: Messenger RNA vaccines lack specificity for dendritic cells (DCs)—the most effective cells at antigen presentation.. Here we report the design and performance of a DC-targeting virus-like particle pseudotyped with an engineered Sindbis-virus glycoprotein that recognizes a surface protein on DCs, and packaging mRNA encoding for the Spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or for the glycoproteins B and D of herpes simplex virus 1.. Injection of the DC-targeting SARS-CoV-2 mRNA vaccine in the footpad of mice led to substantially higher and durable antigen-specific immunoglobulin-G titres and cellular immune responses than untargeted virus-like particles and lipid–nanoparticle formulations.

Article Title: A single dose SARS-CoV-2 simulating particle vaccine induces potent neutralizing activities
Article Snippet: .. Huh-7 cells were subsequently lysed with 50 μL lysis reagent (Promega), and 30 μL lysates were transferred to 96-well Costar flat-bottom luminometer plates (Corning Costar) for the detection of relative light units using the Firefly Luciferase Assay Kit (Promega) with an Ultra 384 luminometer (Tecan). .. A nonlinear regression analysis was performed on the resulting curves using Prism (GraphPad) to calculate half-maximal inhibitory concentration (EC50) values.

Article Title: A pan-coronavirus fusion inhibitor targeting the HR1 domain of human coronavirus spike
Article Snippet: .. Twelve hours after infection, culture medium was refreshed and then incubated for an additional 48 hours, followed by washing cells with PBS, lysing cells with lysis reagent (Promega), and transferring the cell lysates to 96-well Costar flat-bottom luminometer plates (Corning Costar) for the detection of relative light units using the Firefly Luciferase Assay Kit (Promega) and an Ultra 384 luminometer (Tecan). ..

Article Title: A potent SARS-CoV-2 antibody neutralizes Omicron variant by disassembling the spike trimer
Article Snippet: .. Huh-7 cells were subsequently lysing with 50 μL lysis reagent (Promega), and 30 μL of the lysates were transferred to 96-well Costar flat-bottom luminometer plates (Corning Costar) for the detection of relative light units using the Firefly Luciferase Assay Kit (Promega) on the instrument of an Ultra luminometer (Tecan). .. A nonlinear regression analysis was performed on the resulting curves using Prism (GraphPad) to calculate half-maximal inhibitory concentration (IC 50 ) values.

Article Title: TCM theory-inspired discovery of DNJ-flavonoid conjugates as broad-spectrum anti-SARS-CoV-2 agents by primarily targeting ER-associated glycoprotein folding process.
Article Snippet: The global COVID-19 pandemic caused by SARS-CoV-2 has underscored the urgent need for the development of new broad-spectrum antivirals to combat its high mutation rate and the emerging variants.. Host ER α-glucosidases I/II are promising host-targeted therapeutic targets for the development of broad-spectrum antivirals against viral strains that depend on ERQC for infectivity.. Herein, we designed and synthesized a series of TCM theory-inspired DNJ-flavonoid conjugates as novel α-glucosidase inhibitors, which were screened against their in vitro antiviral activities in non-replicative SARS-CoV-2 pseudovirus (PsV) assay.

Infection:

Article Title: A pan-coronavirus fusion inhibitor targeting the HR1 domain of human coronavirus spike
Article Snippet: .. Twelve hours after infection, culture medium was refreshed and then incubated for an additional 48 hours, followed by washing cells with PBS, lysing cells with lysis reagent (Promega), and transferring the cell lysates to 96-well Costar flat-bottom luminometer plates (Corning Costar) for the detection of relative light units using the Firefly Luciferase Assay Kit (Promega) and an Ultra 384 luminometer (Tecan). ..

Article Title: TCM theory-inspired discovery of DNJ-flavonoid conjugates as broad-spectrum anti-SARS-CoV-2 agents by primarily targeting ER-associated glycoprotein folding process.
Article Snippet: The global COVID-19 pandemic caused by SARS-CoV-2 has underscored the urgent need for the development of new broad-spectrum antivirals to combat its high mutation rate and the emerging variants.. Host ER α-glucosidases I/II are promising host-targeted therapeutic targets for the development of broad-spectrum antivirals against viral strains that depend on ERQC for infectivity.. Herein, we designed and synthesized a series of TCM theory-inspired DNJ-flavonoid conjugates as novel α-glucosidase inhibitors, which were screened against their in vitro antiviral activities in non-replicative SARS-CoV-2 pseudovirus (PsV) assay.

Incubation:

Article Title: A pan-coronavirus fusion inhibitor targeting the HR1 domain of human coronavirus spike
Article Snippet: .. Twelve hours after infection, culture medium was refreshed and then incubated for an additional 48 hours, followed by washing cells with PBS, lysing cells with lysis reagent (Promega), and transferring the cell lysates to 96-well Costar flat-bottom luminometer plates (Corning Costar) for the detection of relative light units using the Firefly Luciferase Assay Kit (Promega) and an Ultra 384 luminometer (Tecan). ..

Article Title: TCM theory-inspired discovery of DNJ-flavonoid conjugates as broad-spectrum anti-SARS-CoV-2 agents by primarily targeting ER-associated glycoprotein folding process.
Article Snippet: The global COVID-19 pandemic caused by SARS-CoV-2 has underscored the urgent need for the development of new broad-spectrum antivirals to combat its high mutation rate and the emerging variants.. Host ER α-glucosidases I/II are promising host-targeted therapeutic targets for the development of broad-spectrum antivirals against viral strains that depend on ERQC for infectivity.. Herein, we designed and synthesized a series of TCM theory-inspired DNJ-flavonoid conjugates as novel α-glucosidase inhibitors, which were screened against their in vitro antiviral activities in non-replicative SARS-CoV-2 pseudovirus (PsV) assay.

Transferring:

Article Title: A pan-coronavirus fusion inhibitor targeting the HR1 domain of human coronavirus spike
Article Snippet: .. Twelve hours after infection, culture medium was refreshed and then incubated for an additional 48 hours, followed by washing cells with PBS, lysing cells with lysis reagent (Promega), and transferring the cell lysates to 96-well Costar flat-bottom luminometer plates (Corning Costar) for the detection of relative light units using the Firefly Luciferase Assay Kit (Promega) and an Ultra 384 luminometer (Tecan). ..

Article Title: TCM theory-inspired discovery of DNJ-flavonoid conjugates as broad-spectrum anti-SARS-CoV-2 agents by primarily targeting ER-associated glycoprotein folding process.
Article Snippet: The global COVID-19 pandemic caused by SARS-CoV-2 has underscored the urgent need for the development of new broad-spectrum antivirals to combat its high mutation rate and the emerging variants.. Host ER α-glucosidases I/II are promising host-targeted therapeutic targets for the development of broad-spectrum antivirals against viral strains that depend on ERQC for infectivity.. Herein, we designed and synthesized a series of TCM theory-inspired DNJ-flavonoid conjugates as novel α-glucosidase inhibitors, which were screened against their in vitro antiviral activities in non-replicative SARS-CoV-2 pseudovirus (PsV) assay.

Activity Assay:

Article Title: ADS-J1 inhibits HIV-1 infection and membrane fusion by targeting the highly conserved pocket in the gp41 NHR-trimer.
Article Snippet: The cells were washed with PBS and lysed using the lysis reagent from a luciferase kit (Promega, Madison, WI). .. The cell lysates were transferred to 96-well Costar flat-bottom luminometer plates (Corning Costar, Corning, NY) and luciferase substrate (Promega) was added to the plate, followed by immediate measuring for luciferase activity (relative light units, RLU) using an Ultra 384 luminometer (Tecan, USA). .. To construct vectors encoding N36Fd and its mutants, the corresponding DNA fragments in the pseudovirus mutants Q64 (A/L) and A67 (G/S), as well as the T2635-resistant virus carrying a Q66R mutation, were amplified by PCR using the Platinum PCR SuperMix High Fidelity Kit (Invitrogen) with a forward primer containing a BamH1 site and a reverse primer coding the first 6 amino acids of the Fd fragment.



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